The neuroprotective and anticarcinogenic properties of sodium dichloroacetate and potent bioantioxidantpolyphenolson A neuroblastoma cell line
Mentor: Dr. Annemarie Bettica
Presented at Tribeta Regional Northeast-1 Convention
Awarded: Frank G. Brooks Award-1st Place
Abstract
Recent studies have demonstrated that Dichloroacetate (DCA) has promoted apoptosis in lung, breast and glioblastomal cancer cell lines. The small molecule sodium DCA has been in use for many years to treat diseases such as lactic acidosis and inherited defects in mitochondrial metabolism. Its ability to restore mitochondrial function may have neuroprotective function in neurodegenerative disease models as well as anticarcinogenic effect in other cancer cell lines, especially in combination with natural polyphenols such as curcumin and resveratrol. B35 rat neuroblastoma cells (ATCC), which have an approximate doubling time of 24 hours, were maintained in 90:10 DMEM: FBS, and 1% pen/strep in a 95% air/ 5% CO2 humidified incubator. Upon reaching 95-100% confluency, the cells were subcultured or plated using 0.25% trypsin-EDTA. To determine the neuroprotective efficacy of DCA under oxidative and nonoxidative stress, Parkinsonian symptoms were induced using MPP+, a mitochondrial complex I inhibitor or 6-OHDA, a potential ROS generator after being exposed to DCA. To elucidate the anticarcinogenic properties, B-35 cells were treated with DCA. Single treatment studies included exposure of cells to 10-fold dilutions of DCA, while combination treatments involved treating cells with effective doses of DCA and those of curcumin and resveratrol that were determined through dose-response experiments. The inhibition of growth rate and cell viability were determined by the MTT cell proliferation assay. Through a one-way ANOVA followed by Tukey’s and Dunnett’s post-hoc tests, the experimental means were compared to the solvent control, at a 95% confidence level.
From the left:
Turmeric (Curcumin source), Red grapes and wine (resveratrol source), Sodim dichloroacetate